Composite

Part:BBa_K4769420:Design

Designed by: Echo Gilpin   Group: iGEM23_Cambridge   (2023-10-11)


spoIIE Knockout Construct (5' & 3' HR)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1557
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1557
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1557
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1557
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1224


Design Notes

N/A


Source

TBC

References